Journal: Frontiers in Neurology

Article Title: Minocycline partially reverses established PTSD-related behavioral traits in rats exposed to repetitive low-level blast injury

doi: 10.3389/fneur.2025.1666737

Figure Lengend Snippet: Western blot analysis of 5-HT2AR in rats from cohort 2. 5-HT2AR expression was analyzed in the anterior cortex of control rats treated with saline ( n = 5), and blast-exposed rats treated with saline or minocycline ( n = 6). Top panel: 5-HT2AR; middle panel GAPDH; bottom panel: Ponceau S staining. The targets were sequentially analyzed on the same blot in the same order as indicated in the Figure. The bar graphs show 5-HT2AR levels expressed as a ratio to GAPDH or total protein load calculated from the Ponceau S staining. Error bars indicate the SEM in all panels. Asterisks indicate significant differences (Fisher's LSD after a significant one-way ANOVA, * p < 0.05, ** p < 0.01).

Article Snippet: 5-HT2AR , Mouse monoclonal , Santa Cruz , sc-166775 , 1:600.

Techniques: Western Blot, Expressing, Control, Saline, Staining

Journal: Journal of Neurochemistry

Article Title: Serotonergic Psychedelics Rapidly Modulate Evoked Glutamate Release in Cultured Cortical Neurons

doi: 10.1111/jnc.70020

Figure Lengend Snippet: 5‐HT2AR antagonists ketanserin and M100907, as well as the nonselective 5‐HT2AR agonist lisuride, affect SV pools. Representative colour gradient images (range shown in the rectangle below) from live‐cell imaging of sypHy‐expressing neurons treated with vehicle (CTRL) or ketanserin (KTSR) 1 or 5 μM (a), CTRL or M100907 1 or 10 μM (e), and CTRL, serotonin (SER) 10 or 100 μM or lisuride 10 μM (i). Images show cells during baseline recording, stimulation with 40 and 900 APs at 20 Hz and upon NH 4 Cl pulse, in the presence of bafilomycin A1. Scale bar is 10 μM. (b, f, j) Average traces of sypHy fluorescence intensity normalised to minimum (baseline, F 0 ) and maximum (NH 4 Cl pulse, F max ). Total number of recordings per treatment from 3 independent culture preparations is indicated in brackets. Quantification of the RRP (c, g, k) and TRP (d, h, l) fractions of SVs from the respective experiments. In graphs, bars show the mean, whiskers SEM and circles all data points. Statistical significance was assessed by one‐way ANOVA (KTSR: RRP: F (2,24) = 3.294, p = 0.054, TRP: F (2,24) = 21.59, p < 0.001; M100907: RRP: F (2,28) = 3.476, p = 0.045, TRP: F (2,28) = 4.757, p = 0.017; SER + LIS: RRP: F (3,48) = 0.353, p = 0.787, TRP: F (3,48) = 16.12, p < 0.001) with Dunnett's multiple comparisons test CTRL versus each drug (KTSR: TRP: 1 μM p = 0.676, 5 μM p < 0.001; M100907: RRP: 1 μM p = 0.212, 10 μM p = 0.029, TRP: 1 μM p = 0.242, 10 μM p = 0.010; SER + LIS: TRP: SER 10 μM p = 0.829, SER 100 μM p = 0.189, LIS p < 0.001).

Article Snippet: For treatment of cells, the following drugs were used: lysergic acid diethylamide (LSD) fumarate (custom synthesis, Alfarma), N,N ‐dimethyltryptamine (DMT) fumarate (cat. no. 9003568, Cayman Chemical), 4‐hydroxydimethyltryptamine (psilocin, cat. no. 11864, Cayman Chemical), all diluted in 5% DMSO; 5‐HT2AR antagonists M100907 (volinanserin; cat. no. T5389, TargetMol) diluted in DMSO and ketanserin tartrate (cat. no. AG‐CR1‐0014‐M010, AdipoGen Life Sciences) in 2.5% DMSO, serotonin hydrochloride (cat. no. H9523, Sigma‐Aldrich) in H 2 O, lisuride maleate (cat. no. 4052, Tocris), 5‐HT7 rec.

Techniques: Live Cell Imaging, Expressing, Fluorescence